Macrophage uptake and retention of radiolabeled glycopeptidolipid antigens associated with the superficial L1 layer of Mycobacterium intracellulare serovar 20
نویسندگان
چکیده
منابع مشابه
Macrophage uptake and retention of radiolabeled glycopeptidolipid antigens associated with the superficial L1 layer of Mycobacterium intracellulare serovar 20.
Glycopeptidolipid (GPL) antigens which are associated with the superficial L1 layer of Mycobacterium intracellulare serovar 20 were labeled with radioisotopes by means of internal labeling techniques and used in macrophage uptake and retention studies. The use of tritiated alanine and phenylalanine allowed the incorporation of label into the GPL invariant fatty acyl peptide core, which is commo...
متن کاملPostphagocytic detection of glycopeptidolipids associated with the superficial L1 layer of Mycobacterium intracellulare.
The superficial L1 layer of Mycobacterium intracellulare serovar 20 was detected by immunocytochemical procedures based on C-mycoside glycopeptidolipid (GPL) antigens. Specific immune serum was produced by injecting a GPL-methylated bovine serum albumin complex into rabbits with Freund incomplete adjuvant. The resulting antibodies were used to label serovar 20 with goat anti-rabbit immunoglobul...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1986
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.54.1.133-141.1986